Saturday, April 11, 2009

Gene Function of p30 in Mycoplasma Pneumonia

  • Mycoplasma pneumoniae gene, p30 code for adhesin protein which associated with the attachment organelle of Mycoplasma pneumoniae, yet, its deep function is not clear.
  • Needed for cytadherence (will attach Mycoplasma to epithelium) and it is virulence which will cause disease.
  • P30 is a 29.7-kDa polypeptide, positively charged amino terminus and is followed by a hydrophobic domain of 23 residues which may act as a signal peptide.
  • A second hydrophobic domain follows 40 residues after the first.
  • However, P30 is predicted as highly hydrophilic.
  • The C-terminal domain of P30 shows important sequence homology with the C terminus of the adhesin P1.
  • P30 exhibits immunological cross-reactivity with fibrinogen, keratin, and myosin, which might used for autoimmune affect associated with Mycoplasma pneumoniae infections.
  • Inability to cytadhere happened due to a complete loss of P30 (mutant II-3) or a 144-bp deletion near the 3′ hydroxyl end of the p30 gene (mutant II-7).
  • Monoclonal antibody is used directly to against the extracellular domain of P30 which block its adherence, but this may reflect stearic interference with adjacent molecules on the Mycoplasma surface.